Protein interactions in situ

Discovering Domains Mediating Protein Interactions

Background: Protein-protein interactions do not provide any direct information re‌garding the domains within the proteins that mediate the interactions. The majority of proteins are multi domain proteins and the interaction between them is often defined by the pairs of their domains. Most of the former studies focus only on interacting do‌main pairs. However they do not consider the in...

Visualising individual sequence-specific protein-DNA interactions in situ.

Gene expression - a key feature for modulating cell fate-is regulated in part by histone modifications, which modulate accessibility of the chromatin to transcription factors. Until now, protein-DNA interactions (PDIs) have mostly been studied in bulk without retrieving spatial information from the sample or with poor sequence resolution. New tools are needed to reveal proteins interacting with...

In Situ Evaluation of Estrogen Receptor Dimers in Breast Carcinoma Cells: Visualization of Protein-Protein Interactions

The estrogen receptor (ER) functions as a dimer and is involved in several different biological functions. However ER dimeric proteins have not been identified by in situ methodologies. Structured illumination microscopy (SIM) has been recently developed, which enabled the localization of protein and protein interaction. Therefore, in this study, we firstly demonstrated that ERs formed both hom...

Comparison of in vitro and in situ techniques for estimating protein degradability of selected feedstuffs

The objective of this study was to estimate the protein degradability parameters of 6 common ruminant feedstuff using the nylon bags (in situ) and the innovative in vitro method-the DaisyII incubator. Significant differences were observed between the in situ and in vitro methods in estimation of the rapidly soluble (a) fraction (Pin vitro method were highly correlated with the estimated values ...

In situ dry matter and crude protein degradability in corn,wheat and millet grain